Posted: September 16th, 2015

Determination of the pka values of Histidine

Determination of the pka values of Histidine

Introduction:
Write about a half page of background about pKa and buffering capacity. In addition to the text introduction, create your own histidine speciation figure using ChemDraw or Chemdoodle, a free molecular drawing website. Place this figure in your introduction section and use it to explain the theory behind the experiment.

Methods:
Write a concise methods section is passive voice. Use sentences like: “10M NaOH was added in 10 microliter increments.” Be sure to include all information that is needed to repeat the experiment, but do not include irrelevant or obvious information. The concentrations and amounts of the reagents are very important and must be included. Results:
Begin the results section by pasting in the completed titration figure. Write a concise figure caption. Then write several paragraphs of text explaining your results. Be sure to estimate the pKa’s of the three ionizable groups of histidine, as indicated on your graph. You should also estimate the NaOH equivalents that were added, based on the equation above. Steep portions of your titration curve should represent whole-number molar equivalents of NaOH, while the shallow portions of your titration curve should represent half molar equivalents.

Discussion:
In a few paragraphs, interpret your results based on the theory outlined in your discussion. Here, you may interpret the meaning of the shape of the titration graph, explaining what the steep and shallow slopes mean. You may also discuss any discrepancies in your data and the theory.

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