Posted: August 25th, 2013
TOTAL = 10 marks
FORMAT OF THE ASSIGNMENT
Please present the assignment as follows:-
(1) Use a header ” Assignment (TilleY)”
Then in the main Lody of the assignment include the following:-
(2) Student number
{3) Paper title {as above), authors, journal, volume and page numbers
(4) Under the title “lntroduction” provide a short summary of the paper (4-5 lines) in your own
words (do NOT plagiarize the abstract of the paper)
– 1ot2′
(5) Under the title “Results” show the associated PyMol irnage of the p-opiod GPCR as
instructed in steps (i) to (iii) below. lnsert this into your assignment document as a PNG file {with a
white background) and label with a detailed figure legend (including the PDB lDs) as “Figure 1″.
(6) Under the title “Discussion” answer the questions in Part C above.
PyMol instructions
ti) Open 4DKL and show {S) the structure in cartoon image and hide (H) all non-bonded atoms
and lines.
(ii) Create a cartoon image of the ligand-bound structure.
{iii) Now select the ligand and show as spheres. [HINT: Select the sequence of the ligand bound
structure and using the “selecting” option in the bottom right hand corner of the viewing screen,
select “Molecules” mode.]
How to make an image in PyMol.
Once you have your structure positioned as you like it:-
i)type in the command line: “ray”
ii) without moving the image, go to the menu “file” -> “Save lmage” -> give the file a name and
save it in a directory of your choice
iii) The file is a png file which can be inserted into a document file.
1. Rosenbaum, D. M., Rasmussen, S. G., and Kobilka, B. K. (2009) The structure and function
of G-protein-coupled receptors, Natu re 459, 35G363.
2. Rosenbaum, D. M., Cherezov, V., Hanson, M. A., Rasmussen, S. G’, Thian, F. S., Kobilka, TS.,
Choi, H. J., Yao, X. J., Weis, W. I., Stevens, R. C., and Kobilka, B. K.(2007) GPCR engineering
yields high-resolution structural insights into beta2-adrenergic receptor function, Science 378,
L266-t273.ORDER WITH US FOR AN A+ QUALITY PAPER….
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