Posted: September 13th, 2017

Rat Ileum – Concentration-Response Relationship and Competitive Antagonism

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Text must be in Arial, 10 point, 1.5 lines spacing with margins of at least 2.5 com all around the text. All supporting material such as tables figures must be in page limit. Graphs should be produced electronically using SPSS, Excel or Graph File. Lab report should be in PDF FILE as well. 

Summary – in this experiment we measure the response to the agonist, methacholine, at muscarinic acetylcholine receptors present on the smooth muscle cells in an ileum preparation. We apply increasing concentrations of methacholine to a strip of ileum in a tissue bath and measure the resulting changes in tension. We construct a concentration-response curve by plotting the response against the concentration of methacholine. We use atropine, a competitive antagonist at muscarinic acetylcholine receptors. I will send the necessary about the guidelines and experiments.

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