Posted: August 19th, 2017

The Restriction enzyme (RE) of E.Coli, Eco.R1 cut the exogenous DNA to produce staggered ends. RE will not cut the endogenous DNA of bacteria because

The Restriction enzyme (RE) of E.Coli, Eco.R1 cut the exogenous DNA to produce staggered ends. RE will
not cut the endogenous DNA of bacteria because
a. it is not freely present in cell b. it is an inactive enzyme c. DNA of bacteria is resistant to RE
d. some sequences of the restriction sites are methylated

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