Posted: September 13th, 2017
Paper, Order, or Assignment Requirements
Measuring the intracellular proliferation of Salmonella
Salmonella enterica serovar Typhimurium is a Gram-negative pathogen that is a major cause of food-acquired gastroenteritis. One key characteristic of S. Typhimurium is its ability to survive and replicate within macrophages, white blood cells that normally function to destroy invasive pathogens. This practical is designed to quantify this intracellular proliferative capacity and test how it is modified in a mutant strain.
Salmonella Survival Assay: 24 well plate
Day before (done for you)
24-well plate layout:
Strain 1
T0 |
Strain 2
T0 |
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Strain 1
T16 |
Strain 2
T16 |
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Strain 1
Reserve well |
Strain 2
Reserve well |
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Day 1 (Wednesday 4th Feb)
Bacterial preparation
INPUT dilution
End of infection
T0 dilution
Day2 (Thursday 5th Feb)
T16 dilution
The following week
Count the colonies on the most appropriate dilution plate for each sample. Using these data, calculate:
The Write-Up
The write-up of this practical forms part of the continual assessment mark for this project. Your write up should consist of three parts:
Part A (<500 words)
Briefly outline the biology and pathogenesis of Salmonella Typhimurium and describe its intracellular lifestyle.
Part B (<1000 words)
In the form of a brief scientific paper, describe and interpret the practical work and the data you obtained. Briefly introduce the question to be addressed, describe the methods used and use appropriate figures to display your results. Include a short discussion to describe your findings and their implications.
Part C (<500 words)
The Salmonella mutant strain you were provided with carried a deletion in the tolC gene. Briefly describe the function of tolC and how this function may relate to intracellular pathogenesis in this organism
Place an order in 3 easy steps. Takes less than 5 mins.